Ontology highlight
ABSTRACT:
SUBMITTER: Gu W
PROVIDER: S-EPMC2776052 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Gu Weifeng W Shirayama Masaki M Conte Darryl D Vasale Jessica J Batista Pedro J PJ Claycomb Julie M JM Moresco James J JJ Youngman Elaine M EM Keys Jennifer J Stoltz Matthew J MJ Chen Chun-Chieh G CC Chaves Daniel A DA Duan Shenghua S Kasschau Kristin D KD Fahlgren Noah N Yates John R JR Mitani Shohei S Carrington James C JC Mello Craig C CC
Molecular cell 20091001 2
Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the Dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) comp ...[more]