Ontology highlight
ABSTRACT:
SUBMITTER: Sugiyama M
PROVIDER: S-EPMC2779202 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Sugiyama Mayu M Sakaue-Sawano Asako A Iimura Tadahiro T Fukami Kiyoko K Kitaguchi Tetsuya T Kawakami Koichi K Okamoto Hitoshi H Higashijima Shin-ichi S Miyawaki Atsushi A
Proceedings of the National Academy of Sciences of the United States of America 20091118 49
By exploiting the cell-cycle-dependent proteolysis of two ubiquitination oscillators, human Cdt1 and geminin, which are the direct substrates of SCF(Skp2) and APC(Cdh1) complexes, respectively, Fucci technique labels mammalian cell nuclei in G(1) and S/G(2)/M phases with different colors. Transgenic mice expressing these G(1) and S/G(2)/M markers offer a powerful means to investigate the coordination of the cell cycle with morphogenetic processes. We attempted to introduce these markers into zeb ...[more]