Real-time prediction of cell division timing in developing zebrafish embryo.

ABSTRACT: Combination of live-imaging and live-manipulation of developing embryos in vivo provides a useful tool to study developmental processes. Identification and selection of target cells for an in vivo live-manipulation are generally performed by experience- and knowledge-based decision-making of the observer. Computer-assisted live-prediction method would be an additional approach to facilitate the identification and selection of the appropriate target cells. Herein we report such a method using developing zebrafish embryos. We choose V2 neural progenitor cells in developing zebrafish embryo as their successive shape changes can be visualized in real-time in vivo. We developed a relatively simple mathematical method of describing cellular geometry of V2 cells to predict cell division-timing ba

SUBMITTER: Kozawa S 

PROVIDER: S-EPMC5011986 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature