Ontology highlight
ABSTRACT:
SUBMITTER: Wen D
PROVIDER: S-EPMC2781684 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Wen Dingyi D Vecchi Malgorzata M MM Gu Sheng S Su Lihe L Dolnikova Jana J Huang Yao-Ming YM Foley Susan F SF Garber Ellen E Pederson Nels N Meier Werner W
The Journal of biological chemistry 20090925 47
Misincorporation of amino acids in proteins expressed in Escherichia coli has been well documented but not in proteins expressed in mammalian cells under normal recombinant protein production conditions. Here we report for the first time that Ser can be incorporated at Asn positions in proteins expressed in Chinese hamster ovary cells. This misincorporation was discovered as a result of intact mass measurement, peptide mapping analysis, and tandem mass spectroscopy sequencing. Our analyses showe ...[more]