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Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.


ABSTRACT: Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non-mAb therapeutic glycoproteins is more likely to reduce production costs significantly. The aim of this study was to establish a versatile target gene screening platform for improving productivity for primarily non-mAb glycoproteins with complete interchangeability of model proteins and target genes using transient expression. The platform consists of four techniques compatible with 96-well microplates: lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation. We were able to demonstrate growth profiles and volumetric productivity of CHO cells in 96-half-deepwell microplates comparable with those obtained in shake flasks. In addition, we demonstrate that split-GFP complementation can be used to accurately measure relative titers of therapeutic glycoproteins. Using this platform, we were able to detect target gene-specific increase in titer and specific productivity of two non-mAb glycoproteins. In conclusion, the platform provides a novel miniaturized and parallelisable solution for screening target genes and holds the potential to unravel genes that can enhance the secretory capacity of CHO cells.

SUBMITTER: Hansen HG 

PROVIDER: S-EPMC4676018 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.

Hansen Henning Gram HG   Nilsson Claes Nymand CN   Lund Anne Mathilde AM   Kol Stefan S   Grav Lise Marie LM   Lundqvist Magnus M   Rockberg Johan J   Lee Gyun Min GM   Andersen Mikael Rørdam MR   Kildegaard Helene Faustrup HF  

Scientific reports 20151211


Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non-mAb therapeutic glycoproteins is more likely to reduce production costs significantly. The aim of this study was to establish a versatile target gene screening platform for improving productivity for primarily non-mAb glycoproteins with complete i  ...[more]

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