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Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells.

ABSTRACT: BACKGROUND:The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. METHODS:In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-hIL-7 recombinant by electroporation. Expression of rhIL-7 in CHO-K1 cells was confirmed by enzyme-linked immunosorbent assay (ELISA) and dot and western blots. RESULTS:On western blots of transformed cells, a single 25 kDa band was observed, consistent with the expected molecular weight of glycosylated hIL-7. No significant expression difference was observed between cells transfected with circular or linear plasmids. CONCLUSION:We established a stable CHO-K1 cell line expressing rhIL-7, which we consider to be a promising system for the production of rhIL-7 as a biopharmaceutical.

SUBMITTER: Toghraie FS 

PROVIDER: S-EPMC5643452 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells.

Toghraie Fatemeh Sadat FS   Sharifzadeh Seyedeh Masoumeh SM   Ramezani Amin A   Mahmoudi Maymand Elham E   Yazdanpanah-Samani Mahsa M   Ghaderi Abbas A  

Reports of biochemistry & molecular biology 20171001 1

<h4>Background</h4>The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia.<h4>Methods</h4>In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-h<i>IL</i>-7 recombinant by electroporation.  ...[more]

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