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ABSTRACT:
SUBMITTER: Manosas M
PROVIDER: S-EPMC2784132 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Manosas Maria M Spiering Michelle M MM Zhuang Zhihao Z Benkovic Stephen J SJ Croquette Vincent V
Nature chemical biology 20091018 12
The unwinding and priming activities of the bacteriophage T4 primosome, which consists of a hexameric helicase (gp41) translocating 5' to 3' and an oligomeric primase (gp61) synthesizing primers 5' to 3', have been investigated on DNA hairpins manipulated by a magnetic trap. We find that the T4 primosome continuously unwinds the DNA duplex while allowing for primer synthesis through a primosome disassembly mechanism or a new DNA looping mechanism. A fused gp61-gp41 primosome unwinds and primes D ...[more]