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Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements.

ABSTRACT: This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide.

SUBMITTER: Lahdesmaki I

PROVIDER: S-EPMC2790816 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements.

Lähdesmäki Ilkka I Chocholous Petr P Carroll Andrea D AD Anderson Judy J Rabinovitch Peter S PS Ruzicka Jaromir J

The Analyst 20090427 7

This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density a ...[more]

PMID: 19562221

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