Ontology highlight
ABSTRACT:
SUBMITTER: Pierce NW
PROVIDER: S-EPMC2791906 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Pierce Nathan W NW Kleiger Gary G Shan Shu-ou SO Deshaies Raymond J RJ
Nature 20091201 7273
The pathway by which ubiquitin chains are generated on substrate through a cascade of enzymes consisting of an E1, E2 and E3 remains unclear. Multiple distinct models involving chain assembly on E2 or substrate have been proposed. However, the speed and complexity of the reaction have precluded direct experimental tests to distinguish between potential pathways. Here we introduce new theoretical and experimental methodologies to address both limitations. A quantitative framework based on product ...[more]