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Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale.

ABSTRACT: Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.

SUBMITTER: Juette MF

PROVIDER: S-EPMC4814340 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale.

Juette Manuel F MF Terry Daniel S DS Wasserman Michael R MR Altman Roger B RB Zhou Zhou Z Zhao Hong H Blanchard Scott C SC

Nature methods 20160215 4

Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscu ...[more]

PMID: 26878382

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