Ontology highlight
ABSTRACT:
SUBMITTER: Bajrami B
PROVIDER: S-EPMC2795074 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Bajrami Besnik B Zhao Linlin L Schenkman John B JB Rusling James F JF
Analytical chemistry 20091201 24
Silica nanoparticle bioreactors featuring thin films of enzymes and polyions were utilized in a novel high-throughput 96-well plate format for drug metabolism profiling. The utility of the approach was illustrated by investigating the metabolism of the drugs diclofenac (DCF), troglitazone (TGZ), and raloxifene, for which we observed known metabolic oxidation and bioconjugation pathways and turnover rates. A broad range of enzymes was included by utilizing human liver (HLM), rat liver (RLM) and b ...[more]