Ontology highlight
ABSTRACT:
SUBMITTER: Neubauer C
PROVIDER: S-EPMC2807027 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Neubauer Cajetan C Gao Yong-Gui YG Andersen Kasper R KR Dunham Christine M CM Kelley Ann C AC Hentschel Jendrik J Gerdes Kenn K Ramakrishnan V V Brodersen Ditlev E DE
Cell 20091201 6
Translational control is widely used to adjust gene expression levels. During the stringent response in bacteria, mRNA is degraded on the ribosome by the ribosome-dependent endonuclease, RelE. The molecular basis for recognition of the ribosome and mRNA by RelE and the mechanism of cleavage are unknown. Here, we present crystal structures of E. coli RelE in isolation (2.5 A) and bound to programmed Thermus thermophilus 70S ribosomes before (3.3 A) and after (3.6 A) cleavage. RelE occupies the A ...[more]