Ontology highlight
ABSTRACT:
SUBMITTER: Perla-Kajan J
PROVIDER: S-EPMC2816605 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Perla-Kajan Joanna J Lin Xin X Cooperman Barry S BS Goldman Emanuel E Jakubowski Hieronim H Knudsen Charlotte R CR Mandecki Wlodek W
Protein engineering, design & selection : PEDS 20100118 3
Here we describe the design, preparation and characterization of 10 EF-Tu mutants of potential utility for the study of Escherichia coli elongation factor Tu (EF-Tu) interaction with tRNA by a fluorescence resonance energy transfer assay. Each mutant contains a single cysteine residue at positions in EF-Tu that are proximal to tRNA sites within the aminoacyl-tRNA.EF-Tu.GTP ternary complex that have previously been labeled with fluorophores. These positions fall in the 323-326 and 344-348 regions ...[more]