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Transcriptional control of stem cell maintenance in the Drosophila intestine.

ABSTRACT: Adult stem cells maintain tissue homeostasis by controlling the proper balance of stem cell self-renewal and differentiation. The adult midgut of Drosophila contains multipotent intestinal stem cells (ISCs) that self-renew and produce differentiated progeny. Control of ISC identity and maintenance is poorly understood. Here we find that transcriptional repression of Notch target genes by a Hairless-Suppressor of Hairless complex is required for ISC maintenance, and identify genes of the Enhancer of split complex [E(spl)-C] as the major targets of this repression. In addition, we find that the bHLH transcription factor Daughterless is essential to maintain ISC identity and that bHLH binding sites promote ISC-specific enhancer activity. We propose that Daughterless-dependent bHLH activity is important for the ISC fate and that E(spl)-C factors inhibit this activity to promote differentiation.

SUBMITTER: Bardin AJ

PROVIDER: S-EPMC2827683 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Transcriptional control of stem cell maintenance in the Drosophila intestine.

Bardin Allison J AJ Perdigoto Carolina N CN Southall Tony D TD Brand Andrea H AH Schweisguth François F

Development (Cambridge, England) 20100301 5

Adult stem cells maintain tissue homeostasis by controlling the proper balance of stem cell self-renewal and differentiation. The adult midgut of Drosophila contains multipotent intestinal stem cells (ISCs) that self-renew and produce differentiated progeny. Control of ISC identity and maintenance is poorly understood. Here we find that transcriptional repression of Notch target genes by a Hairless-Suppressor of Hairless complex is required for ISC maintenance, and identify genes of the Enhancer ...[more]

PMID: 20147375

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