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High-speed, miniaturized fluorescence microscopy in freely moving mice.


ABSTRACT: A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca(2+) dynamics.

SUBMITTER: Flusberg BA 

PROVIDER: S-EPMC2828344 | biostudies-literature |

REPOSITORIES: biostudies-literature

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