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High-speed microscopy of continuously moving cell culture vessels.

ABSTRACT: We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

SUBMITTER: Schenk FW

PROVIDER: S-EPMC5036042 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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High-speed microscopy of continuously moving cell culture vessels.

Schenk Friedrich Walter FW Brill Nicolai N Marx Ulrich U Hardt Daniel D König Niels N Schmitt Robert R

Scientific reports 20160926

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus syst ...[more]

PMID: 27667637

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