A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.
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ABSTRACT:
SUBMITTER: Gonzalez-Vera JA
PROVIDER: S-EPMC2838374 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.
González-Vera Juan A JA Luković Elvedin E Imperiali Barbara B
Bioorganic & medicinal chemistry letters 20081227 4
A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature. ...[more]
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