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A photoactivatable green-fluorescent protein from the phylum Ctenophora.

ABSTRACT: Genes for the family of green-fluorescent proteins (GFPs) have been found in more than 100 species of animals, with some species containing six or more copies producing a variety of colours. Thus far, however, these species have all been within three phyla: Cnidaria, Arthropoda and Chordata. We have discovered GFP-type fluorescent proteins in the phylum Ctenophora, the comb jellies. The ctenophore proteins share the xYG chromophore motif of all other characterized GFP-type proteins. These proteins exhibit the uncommon property of reversible photoactivation, in which fluorescent emission becomes brighter upon exposure to light, then gradually decays to a non-fluorescent state. In addition to providing potentially useful optical probes with novel properties, finding a fluorescent protein in one of the earliest diverging metazoans adds further support to the possibility that these genes are likely to occur throughout animals.

SUBMITTER: Haddock SH

PROVIDER: S-EPMC2842807 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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A photoactivatable green-fluorescent protein from the phylum Ctenophora.

Haddock Steven H D SH Mastroianni Nadia N Christianson Lynne M LM

Proceedings. Biological sciences 20091216 1685

Genes for the family of green-fluorescent proteins (GFPs) have been found in more than 100 species of animals, with some species containing six or more copies producing a variety of colours. Thus far, however, these species have all been within three phyla: Cnidaria, Arthropoda and Chordata. We have discovered GFP-type fluorescent proteins in the phylum Ctenophora, the comb jellies. The ctenophore proteins share the xYG chromophore motif of all other characterized GFP-type proteins. These protei ...[more]

PMID: 20018790

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