Dataset Information

Versatile virus-like particle carrier for epitope based vaccines.

ABSTRACT:

Background

Recombinant proteins and in particular single domains or peptides are often poorly immunogenic unless conjugated to a carrier protein. Virus-like-particles are a very efficient means to confer high immunogenicity to antigens. We report here the development of virus-like-particles (VLPs) derived from the RNA bacteriophage AP205 for epitope-based vaccines.

Methodology/principal findings

Peptides of angiotensin II, S.typhi outer membrane protein (D2), CXCR4 receptor, HIV1 Nef, gonadotropin releasing hormone (GnRH), Influenza A M2-protein were fused to either N- or C-terminus of AP205 coat protein. The A205-peptide fusions assembled into VLPs, and peptides displayed on the VLP were highly immunogenic in mice. GnRH fused to the C-terminus of AP205 induced a strong antibody response that inhibited GnRH function in vivo. Exposure of the M2-protein peptide at the N-terminus of AP205 resulted in a strong M2-specific antibody response upon immunization, protecting 100% of mice from a lethal influenza infection.

Conclusions/significance

AP205 VLPs are therefore a very efficient and new vaccine system, suitable for complex and long epitopes, of up to at least 55 amino acid residues in length. AP205 VLPs confer a high immunogenicity to displayed epitopes, as shown by inhibition of endogenous GnRH and protective immunity against influenza infection.

SUBMITTER: Tissot AC

PROVIDER: S-EPMC2843720 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Publications

Versatile virus-like particle carrier for epitope based vaccines.

Tissot Alain C AC Renhofa Regina R Schmitz Nicole N Cielens Indulis I Meijerink Edwin E Ose Velta V Jennings Gary T GT Saudan Philippe P Pumpens Paul P Bachmann Martin F MF

PloS one 20100323 3

<h4>Background</h4>Recombinant proteins and in particular single domains or peptides are often poorly immunogenic unless conjugated to a carrier protein. Virus-like-particles are a very efficient means to confer high immunogenicity to antigens. We report here the development of virus-like-particles (VLPs) derived from the RNA bacteriophage AP205 for epitope-based vaccines.<h4>Methodology/principal findings</h4>Peptides of angiotensin II, S.typhi outer membrane protein (D2), CXCR4 receptor, HIV1 ...[more]

PMID: 20352110

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Dataset Information

Versatile virus-like particle carrier for epitope based vaccines.

Background

Methodology/principal findings

Conclusions/significance

Publications

Versatile virus-like particle carrier for epitope based vaccines.

Similar Datasets

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