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Epitope-Specific Anti-hCG Vaccines on a Virus Like Particle Platform.


ABSTRACT: The possibility of a contraceptive vaccine targeting human chorionic gonadotropin has long been recognized, but never fully realized. Here we describe an epitope-specific approach based on immunogenic display of hCG-derived peptides on virus-like particles of RNA bacteriophage. A number of recombinant VLPs were constructed, each displaying a different hCG-derived peptide. Some were taken from the disordered C-terminal tail of the hormone, another came from an internal loop, and yet another was an epitope mimic produced by affinity-selection on an hCG-neutralizing antibody target. Immunization of mice with some VLPs yielded antisera that bound the hormone and neutralized hCG biological activity.

SUBMITTER: Caldeira J 

PROVIDER: S-EPMC4627648 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Epitope-Specific Anti-hCG Vaccines on a Virus Like Particle Platform.

Caldeira Jerri J   Bustos Jeremiah J   Peabody Julianne J   Chackerian Bryce B   Peabody David S DS  

PloS one 20151030 10


The possibility of a contraceptive vaccine targeting human chorionic gonadotropin has long been recognized, but never fully realized. Here we describe an epitope-specific approach based on immunogenic display of hCG-derived peptides on virus-like particles of RNA bacteriophage. A number of recombinant VLPs were constructed, each displaying a different hCG-derived peptide. Some were taken from the disordered C-terminal tail of the hormone, another came from an internal loop, and yet another was a  ...[more]

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