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Techniques used to study the DNA polymerase reaction pathway.

ABSTRACT: A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.

SUBMITTER: Joyce CM

PROVIDER: S-EPMC2846202 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Techniques used to study the DNA polymerase reaction pathway.

Joyce Catherine M CM

Biochimica et biophysica acta 20090807 5

A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway. ...[more]

PMID: 19665596

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