Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC2855623 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Liu Ying Y Ye Xuecheng X Jiang Feng F Liang Chunyang C Chen Dongmei D Peng Junmin J Kinch Lisa N LN Grishin Nick V NV Liu Qinghua Q
Science (New York, N.Y.) 20090801 5941
The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- and duplex siRNA-initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of ...[more]