Ontology highlight
ABSTRACT:
SUBMITTER: Yuan YR
PROVIDER: S-EPMC4689305 | biostudies-literature | 2005 Aug
REPOSITORIES: biostudies-literature
Yuan Yu-Ren YR Pei Yi Y Ma Jin-Biao JB Kuryavyi Vitaly V Zhadina Maria M Meister Gunter G Chen Hong-Ying HY Dauter Zbigniew Z Tuschl Thomas T Patel Dinshaw J DJ
Molecular cell 20050801 3
Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' ...[more]