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Evaluation of ex vivo human immune response against candidate antigens for a visceral leishmaniasis vaccine.


ABSTRACT: People cured from visceral leishmaniasis (VL) develop protection mediated by Th1-type cellular responses against new infections. We evaluated cytokine responses against 6 defined candidate vaccine antigens in 15 cured VL subjects and 5 healthy endemic controls with no evidence of previous exposure to Leishmania parasites. Of the 6 cytokines examined, only interferon-gamma (IFN-gamma) differentiated cured VL patients from non-exposed individuals, with cured patients mounting a significantly higher IFN-gamma response to a crude parasite antigen preparation. Among candidate vaccine antigens tested, the largest number of cured subjects recognized cysteine proteinase B, leading to heightened IFN-gamma responses, followed by sterol 24-c-methyltransferase. These two antigens were the most immunogenic and protective antigens in a murine VL model, indicating a relationship between T cell recall responses of humans cured from VL and protective efficacy in an experimental model. Further studies may help prioritize antigens for clinical development of a subunit vaccine against VL.

SUBMITTER: Kumar R 

PROVIDER: S-EPMC2861380 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Evaluation of ex vivo human immune response against candidate antigens for a visceral leishmaniasis vaccine.

Kumar Rajiv R   Goto Yasuyuki Y   Gidwani Kamlesh K   Cowgill Karen D KD   Sundar Shyam S   Reed Steven G SG  

The American journal of tropical medicine and hygiene 20100501 5


People cured from visceral leishmaniasis (VL) develop protection mediated by Th1-type cellular responses against new infections. We evaluated cytokine responses against 6 defined candidate vaccine antigens in 15 cured VL subjects and 5 healthy endemic controls with no evidence of previous exposure to Leishmania parasites. Of the 6 cytokines examined, only interferon-gamma (IFN-gamma) differentiated cured VL patients from non-exposed individuals, with cured patients mounting a significantly highe  ...[more]

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