Ontology highlight
ABSTRACT:
SUBMITTER: Calixto A
PROVIDER: S-EPMC2862115 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Calixto Andrea A Ma Charles C Chalfie Martin M
Nature methods 20100404 5
We describe a method for conditional regulation of gene expression based on the processing of an intron cassette. The RNA processing factor MEC-8 is necessary for the function of the Caenorhabditis elegans touch receptor neurons; mec-8 mutants are touch insensitive. We show here that this insensitivity involves the loss of MEC-8-dependent splicing of mec-2, which encodes a component of the mechanosensory transduction complex. MEC-8 is needed to remove the ninth intron in mec-2 pre-mRNA to form t ...[more]