Synthesis and binding studies of two new macrocyclic receptors for the stereoselective recognition of dipeptides.

ABSTRACT: We present here the design, synthesis, and analysis of a series of receptors for peptide ligands inspired by the hydrogen-bonding pattern of protein ?-sheets. The receptors themselves can be regarded as strands 1 and 3 of a three-stranded ?-sheet, with cross-linking between the chains through the 4-position of adjacent phenylalanine residues. We also report on the conformational equilibria of these receptors in solution as well as on their tendency to dimerize. ¹H NMR titration experiments are used to quantify the dimerization constants, as well as the association constant values of the 1:1 complexes formed between the receptors and a series of diamides and dipeptides. The receptors show moderate levels of selectivity in the molecular recognition of the hydrogen-bonding pattern present in the diamide series, selecting the alpha-amino acid-related hydrogen-bonding functionality. Only one of the two cyclic receptors shows modest signs of enantioselectivity and moderate diastereoselectivity in the recognition of the enantiomers and diastereoisomers of the Ala-Ala dipeptide (??G?? (DD-DL) = -1.08 kcal/mol and ??G?? (DD-LD) = -0.89 kcal/mol). Surprisingly, the linear synthetic precursors show higher levels of stereoselectivity than their cyclic counterparts.

SUBMITTER: Castilla AM 

PROVIDER: S-EPMC2870531 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature