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The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors.

ABSTRACT: ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic and biochemical analyses.

SUBMITTER: Arancio W

PROVIDER: S-EPMC2870949 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors.

Arancio Walter W Onorati Maria C MC Burgio Giosalba G Collesano Marianna M Ingrassia Antonia M R AM Genovese Swonild I SI Fanto Manolis M Corona Davide F V DF

Genetics 20100301 1

ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based a ...[more]

PMID: 20194965

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Genetic identification of a network of factors that functionally interact with the nucleosome remodeling ATPase ISWI.

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