Ontology highlight
ABSTRACT:
SUBMITTER: Ma SM
PROVIDER: S-EPMC2875069 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Ma Suzanne M SM Li Jesse W-H JW Choi Jin W JW Zhou Hui H Lee K K Michael KK Moorthie Vijayalakshmi A VA Xie Xinkai X Kealey James T JT Da Silva Nancy A NA Vederas John C JC Tang Yi Y
Science (New York, N.Y.) 20091001 5952
Highly reducing iterative polyketide synthases are large, multifunctional enzymes that make important metabolites in fungi, such as lovastatin, a cholesterol-lowering drug from Aspergillus terreus. We report efficient expression of the lovastatin nonaketide synthase (LovB) from an engineered strain of Saccharomyces cerevisiae, as well as complete reconstitution of its catalytic function in the presence and absence of cofactors (the reduced form of nicotinamide adenine dinucleotide phosphate and ...[more]