Ontology highlight
ABSTRACT:
SUBMITTER: Yuet KP
PROVIDER: S-EPMC7329362 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Yuet Kai P KP Liu Corey W CW Lynch Stephen R SR Kuo James J Michaels Wesley W Lee Robert B RB McShane Abigail E AE Zhong Brian L BL Fischer Curt R CR Khosla Chaitan C
Journal of the American Chemical Society 20200320 13
Several <i>Nocardia</i> strains associated with nocardiosis, a potentially life-threatening disease, house a nonamodular assembly line polyketide synthase (PKS) that presumably synthesizes an unknown polyketide. Here, we report the discovery and structure elucidation of the NOCAP (nocardiosis-associated polyketide) aglycone by first fully reconstituting the NOCAP synthase <i>in vitro</i> from purified protein components followed by heterologous expression in <i>E. coli</i> and spectroscopic anal ...[more]