Dataset Information

Polybasic Nanomatrices Prepared By UV-initiated Photopolymerization.

ABSTRACT: A novel method for synthesizing nanoscale polymer networks that swell in acidic media is described here using photoinitiated emulsion polymerization. These nanomatrices consist of a crosslinked core of poly[2-(diethylamino)ethyl methacrylate] surface grafted with poly(ethylene glycol) (PDGP) with an average diameter of 50-150 nm. Control over mesh size, surface charge, encapsulation efficiency and in vitro biocompatibility was obtained by varying crosslinking density. The ability to image nanomatrices in their dry state using conventional scanning electron microscopy was made possible by increasing crosslinking density. Theoretical calculations of matrix mesh sizes were supported by the encapsulation of both insulin and colloidal gold 2-5 nm in diameter. The ability to sequester and control the aggregation of an inorganic phase confirmed their use as a nanocomposite matrix material. These networks could be used as imaging agents, drug delivery devices or as components of sensing devices.

SUBMITTER: Fisher OZ

PROVIDER: S-EPMC2880552 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Polybasic Nanomatrices Prepared By UV-initiated Photopolymerization.

Fisher Omar Z OZ Peppas Nicholas A NA

Macromolecules 20090501 9

A novel method for synthesizing nanoscale polymer networks that swell in acidic media is described here using photoinitiated emulsion polymerization. These nanomatrices consist of a crosslinked core of poly[2-(diethylamino)ethyl methacrylate] surface grafted with poly(ethylene glycol) (PDGP) with an average diameter of 50-150 nm. Control over mesh size, surface charge, encapsulation efficiency and in vitro biocompatibility was obtained by varying crosslinking density. The ability to image nanoma ...[more]

PMID: 20526378

Similar Datasets

Project description:Photopolymerized biomimetic hydrogels with adaptable properties have been widely used for cell and tissue engineering applications. As a widely adopted gel cross-linking method, photopolymerization provides experimenters on-demand and spatial-temporal controls in gelation kinetics. Long wavelength ultraviolet (UV) light initiated photopolymerization is among the most popular methods in the fabrication of cell-laden hydrogels owing to its rapid and relatively mild gelation conditions. The use of UV light, however, still causes concerns regarding its potential negative impacts on cells. Alternatively, visible light based photopolymerization can be used to cross-link cell-laden hydrogels. The majority of visible light based gelation schemes involve photoinitiator, co-initiator, and comonomer. This multicomponent initiation system creates added challenges for optimizing hydrogel formulations. Here, we report a co-initiator/comonomer-free visible light initiated thiol-norbornene photopolymerization scheme to prepare modular biomimetic hydrogels suitable for in situ cell encapsulation. Eosin-Y was used as the sole initiator to initiate modular gelation between synthetic macromers (e.g., thiolated poly(vinyl alcohol) or poly(ethylene glycol)) and functionalized extracellular matrices (ECMs) including norbornene-functionalized gelatin (GelNB) or thiolated hyaluronic acid (THA). These components are modularly cross-linked to afford bioinert (i.e., purely synthetic), bioactive (i.e., using gelatin), and biomimetic (i.e., using gelatin and hyaluronic acid) hydrogels. The stiffness of the hydrogels can be easily tuned without affecting the contents of the bioactive components. Furthermore, the use of naturally derived biomacromolecules (e.g., gelatin and HA) renders these hydrogels susceptible to enzyme-mediated degradation. In addition to demonstrating efficient and tunable visible light mediated gelation, we also utilized this biomimetic modular gelation system to formulate artificial tumor niche and to study the effects of cell density and gel modulus on the formation of pancreatic ductal adenocarcinoma (PDAC) spheroids.

Dataset Information

Polybasic Nanomatrices Prepared By UV-initiated Photopolymerization.

Publications

Polybasic Nanomatrices Prepared By UV-initiated Photopolymerization.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets