Ontology highlight
ABSTRACT:
SUBMITTER: McQuade LE
PROVIDER: S-EPMC2889334 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20100422 19
We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu(2)(FL2E) and the membran ...[more]