Ontology highlight
ABSTRACT:
SUBMITTER: Dempsey DR
PROVIDER: S-EPMC6093203 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Dempsey Daniel R DR Jiang Hanjie H Kalin Jay H JH Chen Zan Z Cole Philip A PA
Journal of the American Chemical Society 20180723 30
N-Hydroxysuccinimide (NHS)-esters are widely used to label proteins nonselectively on free amino groups. Such broad labeling can be disadvantageous because it can interfere with protein structure or function and because stoichiometry is poorly controlled. Here we describe a simple method to transform NHS-esters into site-specific protein labeling on N-terminal Cys residues. MESNA addition converts NHS-esters to chemoselective thioesters for N-Cys modification. This labeling strategy was applied ...[more]