Multiplex identification of microbes.
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ABSTRACT:
SUBMITTER: Hyman RW
PROVIDER: S-EPMC2893500 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Publications
Multiplex identification of microbes.
Hyman Richard W RW St Onge Robert P RP Allen Edward A EA Miranda Molly M Aparicio Ana Maria AM Fukushima Marilyn M Davis Ronald W RW
Applied and environmental microbiology 20100423 12
We have adapted molecular inversion probe technology to identify microbes in a highly multiplexed procedure. This procedure does not require growth of the microbes. Rather, the technology employs DNA homology twice: once for the molecular probe to hybridize to its homologous DNA and again for the 20-mer oligonucleotide barcode on the molecular probe to hybridize to a commercially available molecular barcode array. As proof of concept, we have designed, tested, and employed 192 molecular probes f ...[more]
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