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Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.


ABSTRACT: Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs ("TiPS") retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respect to morphology, pluripotency-associated marker expression and capacity to generate neurons, cardiomyocytes, and hematopoietic progenitor cells. Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies. Reprogramming T-cells procured in a minimally invasive manner can be used to characterize and expand donor specific iPSCs, and control their differentiation into specific lineages.

SUBMITTER: Brown ME 

PROVIDER: S-EPMC2894062 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.

Brown Matthew E ME   Rondon Elizabeth E   Rajesh Deepika D   Mack Amanda A   Lewis Rachel R   Feng Xuezhu X   Zitur Laura Jo LJ   Learish Randall D RD   Nuwaysir Emile F EF  

PloS one 20100629 6


Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs ("TiPS") retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respe  ...[more]

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