Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative.
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ABSTRACT:
SUBMITTER: Blouzard JC
PROVIDER: S-EPMC2897430 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
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Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative.
Blouzard Jean-Charles JC Valette Odile O Tardif Chantal C de Philip Pascale P
Applied and environmental microbiology 20100430 13
Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome. ...[more]
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