Nucleosome rotational setting is associated with transcriptional regulation in promoters of tissue-specific human genes.

ABSTRACT:

Background

The position of a nucleosome, both translational along the DNA molecule and rotational between the histone core and the DNA, is controlled by many factors, including the regular occurrence of specific dinucleotides with a period of approximately 10 bp, important for the rotational setting of the DNA around the histone octamer.

Results

We show that such a 10 bp periodic signal of purine-purine dinucleotides occurs in phase with the transcription start site (TSS) of human genes and is centered on the position of the first (+1) nucleosome downstream of the TSS. These data support a direct link between transcription and the rotational setting of the nucleosome. The periodic signal is most prevalent in genes that contain CpG islands that are expressed at low levels in

SUBMITTER: Hebert C 

PROVIDER: S-EPMC2898081 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature