Unknown

Dataset Information

0

Epigenetic transcriptional repression of cellular genes by a viral SET protein.


ABSTRACT: Viruses recruit host proteins to secure viral genome maintenance and replication. However, whether they modify host histones directly to interfere with chromatin-based transcription is unknown. Here we report that Paramecium bursaria chlorella virus 1 (PBCV-1) encodes a functional SET domain histone Lys methyltransferase (HKMTase) termed vSET, which is linked to rapid inhibition of host transcription after viral infection. We show that vSET is packaged in the PBCV-1 virion, and that it contains a nuclear localization signal and probably represses host transcription by methylating histone H3 at Lys 27 (H3K27), a modification known to trigger gene silencing in eukaryotes. We also show that vSET induces cell accumulation at the G2/M phase by recruiting the Polycomb repressive complex CBX8 to the methylated H3K27 site in a heterologous system, vSET-like proteins that have H3K27 methylation activity are conserved in chlorella viruses. Our findings suggest a viral mechanism to repress gene transcription by direct modification of chromatin by PBCV-1 vSET.

SUBMITTER: Mujtaba S 

PROVIDER: S-EPMC2898185 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Epigenetic transcriptional repression of cellular genes by a viral SET protein.

Mujtaba Shiraz S   Manzur Karishma L KL   Gurnon James R JR   Kang Ming M   Van Etten James L JL   Zhou Ming-Ming MM  

Nature cell biology 20080901 9


Viruses recruit host proteins to secure viral genome maintenance and replication. However, whether they modify host histones directly to interfere with chromatin-based transcription is unknown. Here we report that Paramecium bursaria chlorella virus 1 (PBCV-1) encodes a functional SET domain histone Lys methyltransferase (HKMTase) termed vSET, which is linked to rapid inhibition of host transcription after viral infection. We show that vSET is packaged in the PBCV-1 virion, and that it contains  ...[more]

Similar Datasets

| S-EPMC4703871 | biostudies-literature
| S-EPMC2840145 | biostudies-literature
| S-EPMC5833687 | biostudies-literature
| S-EPMC2937640 | biostudies-literature
| S-EPMC3049204 | biostudies-literature
| S-EPMC3955737 | biostudies-literature
| S-EPMC4639869 | biostudies-literature
| S-EPMC3315731 | biostudies-literature
| S-EPMC1564388 | biostudies-other
| S-EPMC7347541 | biostudies-literature