Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.
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ABSTRACT:
SUBMITTER: Cascioferro A
PROVIDER: S-EPMC2916470 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Publications
Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.
Cascioferro Alessandro A Boldrin Francesca F Serafini Agnese A Provvedi Roberta R Palù Giorgio G Manganelli Riccardo R
Applied and environmental microbiology 20100611 15
Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis. ...[more]
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