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Efficient and simple generation of multiple unmarked gene deletions in Mycobacterium smegmatis.

ABSTRACT: Research on mycobacterial genetics relies heavily on techniques for directed gene mutation, but genetic studies are often hampered by the difficulty of generating gene deletions in mycobacteria. We developed an efficient and improved deletion system, described here in detail, which can be used to construct multiple unmarked recombinants in mycobacteria. We tested this system by using it to sequentially delete four pairs of toxin-antitoxin genes in Mycobacterium smegmatis.

SUBMITTER: Mao XJ

PROVIDER: S-EPMC4789726 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Efficient and simple generation of multiple unmarked gene deletions in Mycobacterium smegmatis.

Mao Xu-Jian XJ Yan Mei-Yi MY Zhu Hui H Guo Xiao-Peng XP Sun Yi-Cheng YC

Scientific reports 20160314

Research on mycobacterial genetics relies heavily on techniques for directed gene mutation, but genetic studies are often hampered by the difficulty of generating gene deletions in mycobacteria. We developed an efficient and improved deletion system, described here in detail, which can be used to construct multiple unmarked recombinants in mycobacteria. We tested this system by using it to sequentially delete four pairs of toxin-antitoxin genes in Mycobacterium smegmatis. ...[more]

PMID: 26972108

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