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Efficient generation of unmarked deletions in Legionella pneumophila.

ABSTRACT: Unmarked gene deletions facilitate studies of Legionella pneumophila multicomponent processes, such as motility and exonuclease activity. For this purpose, FRT-flanked alleles constructed in Escherichia coli using ?-Red recombinase were transferred to L. pneumophila by natural transformation. Resistance cassettes were then efficiently excised using the Flp site-specific recombinase encoded on a plasmid that is readily lost.

SUBMITTER: Bryan A

PROVIDER: S-EPMC3067435 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Efficient generation of unmarked deletions in Legionella pneumophila.

Bryan Andrew A Harada Kaoru K Swanson Michele S MS

Applied and environmental microbiology 20110204 7

Unmarked gene deletions facilitate studies of Legionella pneumophila multicomponent processes, such as motility and exonuclease activity. For this purpose, FRT-flanked alleles constructed in Escherichia coli using λ-Red recombinase were transferred to L. pneumophila by natural transformation. Resistance cassettes were then efficiently excised using the Flp site-specific recombinase encoded on a plasmid that is readily lost. ...[more]

PMID: 21296953

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