Ontology highlight
ABSTRACT:
SUBMITTER: Bryan A
PROVIDER: S-EPMC3067435 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Bryan Andrew A Harada Kaoru K Swanson Michele S MS
Applied and environmental microbiology 20110204 7
Unmarked gene deletions facilitate studies of Legionella pneumophila multicomponent processes, such as motility and exonuclease activity. For this purpose, FRT-flanked alleles constructed in Escherichia coli using λ-Red recombinase were transferred to L. pneumophila by natural transformation. Resistance cassettes were then efficiently excised using the Flp site-specific recombinase encoded on a plasmid that is readily lost. ...[more]