Ontology highlight
ABSTRACT:
SUBMITTER: Schermelleh L
PROVIDER: S-EPMC2916659 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Schermelleh Lothar L Carlton Peter M PM Haase Sebastian S Shao Lin L Winoto Lukman L Kner Peter P Burke Brian B Cardoso M Cristina MC Agard David A DA Gustafsson Mats G L MG Leonhardt Heinrich H Sedat John W JW
Science (New York, N.Y.) 20080601 5881
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional micr ...[more]