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Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.

ABSTRACT: Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

SUBMITTER: Abrahamsson S

PROVIDER: S-EPMC4161287 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.

Abrahamsson Sara S Chen Jiji J Hajj Bassam B Stallinga Sjoerd S Katsov Alexander Y AY Wisniewski Jan J Mizuguchi Gaku G Soule Pierre P Mueller Florian F Dugast Darzacq Claire C Darzacq Xavier X Wu Carl C Bargmann Cornelia I CI Agard David A DA Dahan Maxime M Gustafsson Mats G L MG

Nature methods 20121209 1

Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image. ...[more]

PMID: 23223154

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