Ontology highlight
ABSTRACT:
SUBMITTER: Bian W
PROVIDER: S-EPMC2924624 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Nature protocols 20090924 10
This protocol describes a cell/hydrogel molding method for precise and reproducible biomimetic fabrication of three-dimensional (3D) muscle tissue architectures in vitro. Using a high aspect ratio soft lithography technique, we fabricate polydimethylsiloxane (PDMS) molds containing arrays of mesoscopic posts with defined size, elongation and spacing. On cell/hydrogel molding, these posts serve to enhance the diffusion of nutrients to cells by introducing elliptical pores in the cell-laden hydrog ...[more]