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Efficient reprogramming of adult neural stem cells to monocytes by ectopic expression of a single gene.

ABSTRACT: Neural stem cells have a broad differentiation repertoire during embryonic development and can be reprogrammed to pluripotency comparatively easily. We report that adult neural stem cells can be reprogrammed at very high efficiency to monocytes, a differentiated fate of an unrelated somatic lineage, by ectopic expression of the Ets transcription factor PU.1. The reprogrammed cells display a marker profile and functional characteristics of monocytes and integrate into tissues after transplantation. The failure to reprogram lineage-committed neural cells to monocytes with PU.1 suggests that neural stem cells are uniquely amenable to reprogramming.

SUBMITTER: Forsberg M

PROVIDER: S-EPMC2930460 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Efficient reprogramming of adult neural stem cells to monocytes by ectopic expression of a single gene.

Forsberg Magda M Carlén Marie M Meletis Konstantinos K Yeung Maggie S Y MS Barnabé-Heider Fanie F Persson Mats A A MA Aarum Johan J Frisén Jonas J

Proceedings of the National Academy of Sciences of the United States of America 20100730 33

Neural stem cells have a broad differentiation repertoire during embryonic development and can be reprogrammed to pluripotency comparatively easily. We report that adult neural stem cells can be reprogrammed at very high efficiency to monocytes, a differentiated fate of an unrelated somatic lineage, by ectopic expression of the Ets transcription factor PU.1. The reprogrammed cells display a marker profile and functional characteristics of monocytes and integrate into tissues after transplantatio ...[more]

PMID: 20675585

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