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Single-gene transgenic mouse strains for reprogramming adult somatic cells.


ABSTRACT: We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.

SUBMITTER: Carey BW 

PROVIDER: S-EPMC3048025 | biostudies-literature |

REPOSITORIES: biostudies-literature

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