Efficient and quantitative high-throughput tRNA sequencing.
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ABSTRACT:
SUBMITTER: Zheng G
PROVIDER: S-EPMC4624326 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Publications
Efficient and quantitative high-throughput tRNA sequencing.
Zheng Guanqun G Qin Yidan Y Clark Wesley C WC Dai Qing Q Yi Chengqi C He Chuan C Lambowitz Alan M AM Pan Tao T
Nature methods 20150727 9
Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to in ...[more]
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