Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.
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ABSTRACT:
SUBMITTER: Bar-Shir A
PROVIDER: S-EPMC4485562 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.
Bar-Shir Amnon A Liang Yajie Y Chan Kannie W Y KW Gilad Assaf A AA Bulte Jeff W M JW
Chemical communications (Cambridge, England) 20150301 23
Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene. ...[more]
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