Ontology highlight
ABSTRACT:
SUBMITTER: Coleman B
PROVIDER: S-EPMC2935960 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Coleman Bryony B Rickard Natalie A NA de Silva Michelle G MG Shepherd Robert K RK
Journal of neuroscience methods 20080913 2
Green fluorescent protein (GFP) has been used extensively to label cells in vitro and to track them following their transplantation in vivo. During our studies using the mouse embryonic stem cell line R1 B5-EGFP, we observed variable levels of fluorescence intensity of the GFP within these transfected cells. The variable fluorescence of this protein coupled with the innately autofluorescent nature of several structures within the cochlea collectively made the in vivo identification of these tran ...[more]