Ontology highlight
ABSTRACT:
SUBMITTER: Kim HM
PROVIDER: S-EPMC2937362 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Kim Hong-Man HM Xu Yongbin Y Lee Minho M Piao Shunfu S Sim Se-Hoon SH Ha Nam-Chul NC Lee Kangseok K
Journal of bacteriology 20100625 17
Tripartite efflux pumps found in Gram-negative bacteria are involved in antibiotic resistance and toxic-protein secretion. In this study, we show, using site-directed mutational analyses, that the conserved residues located in the tip region of the alpha-hairpin of the membrane fusion protein (MFP) AcrA play an essential role in the action of the tripartite efflux pump AcrAB-TolC. In addition, we provide in vivo functional data showing that both the length and the amino acid sequence of the alph ...[more]