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A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.

ABSTRACT: The nonsymmetrical spatial distribution of newly synthesized proteins in animal cells plays a central role in many cellular processes. Here, we report that a simple alkene tag, homoallylglycine (HAG), was co-translationally incorporated into a recombinant protein as well as endogenous, newly synthesized proteins in mammalian cells with high efficiency. In conjunction with a photoinduced tetrazole-alkene cycloaddition reaction ("photoclick chemistry"), this alkene tag further served as a bioorthogonal chemical reporter both for the selective protein functionalization in vitro and for a spatiotemporally controlled imaging of the newly synthesized proteins in live mammalian cells. This two-step metabolic alkene tagging-photocontrolled chemical functionalization approach may offer a potentially useful tool to study the role of spatiotemporally regulated protein synthesis in mammalian cells.

SUBMITTER: Song W

PROVIDER: S-EPMC2942984 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.

Song Wenjiao W Wang Yizhong Y Yu Zhipeng Z Vera Claudia I Rivera CI Qu Jun J Lin Qing Q

ACS chemical biology 20100901 9

The nonsymmetrical spatial distribution of newly synthesized proteins in animal cells plays a central role in many cellular processes. Here, we report that a simple alkene tag, homoallylglycine (HAG), was co-translationally incorporated into a recombinant protein as well as endogenous, newly synthesized proteins in mammalian cells with high efficiency. In conjunction with a photoinduced tetrazole-alkene cycloaddition reaction ("photoclick chemistry"), this alkene tag further served as a bioortho ...[more]

PMID: 20666508

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